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Study of genotypes and virB4 secretion gene of Bartonella henselae strains from patients with clinically defined cat scratch disease.

机译：从临床定义为猫抓病的患者中研究汉氏巴尔通体菌株的基因型和virB4分泌基因。

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Bartonella henselae is the causative agent of cat scratch disease (CSD), which usually presents as a self-limiting lymphadenopathy. Occasionally, the bacteria will spread and be responsible for tissue and visceral involvement. Two B. henselae genotypes (genotypes I and II) have been described to be responsible for uncomplicated CSD on the basis of 16S rRNA sequence analysis. A type IV secretion system (T4SS) similar to the virulence-associated VirB system of Agrobacterium tumefaciens was recently identified in the B. henselae Houston-1 genotype I strain. We studied the correlations of the B. henselae genotypes with the clinical presentations and with the presence of T4SS. Isolates originated from CSD patients whose lymph nodes were prospectively analyzed. B. henselae genotype I was identified in 13 of 42 patients (30%). Among these, two teenage twins presented with hepatosplenic CSD and one immunocompetent adult presented with osteomyelitis. Genotype II was detected in 28 of 42 patients (67%), all of whom presented with uncomplicated CSD. The last patient was infected with both genotypes. T4SS was studied by PCR amplification of the virB4 gene. Amplification of virB4 codons 146 to 256, 273 to 357, and 480 to 537 enabled us to detect 66, 90, and 100% of the B. henselae isolates, respectively. Sequence analysis revealed sequence variations that correlated with genotype distribution. Our studies suggest that B. henselae genotype I strains harbor virB4 genes that are different from those harbored by genotype II strains and that genotype I strains might be more pathogenic.
机译：亨氏巴尔通体是猫抓痒病（CSD）的病原体，通常以自限性淋巴结病的形式出现。有时，细菌会扩散并导致组织和内脏受累。在16S rRNA序列分析的基础上，已经描述了两种B. henselae基因型（基因型I和II）负责复杂的CSD。最近在亨氏芽孢杆菌休斯顿-1基因型I菌株中鉴定了类似于与根癌土壤杆菌的毒力相关VirB系统相似的IV型分泌系统（T4SS）。我们研究了B. henselae基因型与临床表现和T4SS的相关性。分离物来自对淋巴结进行了前瞻性分析的CSD患者。在42例患者中有13例（30％）检出了B. henselae基因型。其中，两名少年双胞胎伴有肝脾CSD，一名免疫能力强的成年人伴有骨髓炎。在42例患者中有28例（67％）检测到II型基因，所有患者均伴有单纯性CSD。最后一名患者感染了两种基因型。通过virB4基因的PCR扩增研究了T4SS。 virB4密码子146至256、273至357和480至537的扩增使我们能够分别检测到66％，90％和100％的汉森芽孢杆菌分离株。序列分析揭示了与基因型分布相关的序列变异。我们的研究表明，亨氏芽孢杆菌基因型I菌株携带的virB4基因与基因型II菌株所携带的病毒不同，并且基因型I菌株可能更具致病性。

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